Evolution from a Molecular Perspective
Introduction: Why globular evolution?
Evolution has been a heavily debated issue since Charles Darwin first documented the
theory in 1859. However, until just recently, adaptation at a molecular level has been
overlooked except by the scientific world. Now with the help of modern technology, the
protein sequences of nearly every known living thing have either been established or are
in the process of establishment, and are widely accessible via the internet. With the
knowledge of these sequences, one can actually look at several organisms genetic codes
and point out the similarities. Entire genomes of creatures have been sequenced, and the
human genome project is well underway and ahead of schedule. With this new knowledge
comes worries, for humans, however. What if the information stored in our genes was
available to the public? Would insurance companies and employers base their selections
on these traits? Also, with the total knowledge of every sequence of every amino acid
chain in a person's genome, couldn't a laboratory perceivably re
construct an exact copy of, or clone, that person? These are all issues that will have
to be dealt with in the near future, but for now we need only concern ourselves with the
objective observation of these proteins in our attempt to explain our ever mysterious
origin. As humans, we are the first creatures to question exactly where we came from and
how we got here. Some cling to religious creationism as a means, while others embrace
the evolutionary theory. As of now, and possibly forever, neither can be proven to be
absolute truth with hard facts, and both have their opposing arguments. The point of
this paper being composed is not to attempt to abolish the creationist view, a feat that
at this point seems impossible, but merely to educate those seeking to unravel the
mystery of our forthcoming by pointing out facts that exist in the modern world and that
can be quite easily and independently researched. It is conceivable that the two ideas,
creationism and evolutionism, can exist symbiotically due to t
he fact that both views have very good points.
Hemoglobin: Comparisons between species
Of all the proteins in living things, hemoglobin is "the second most interesting
substance in the world," as American biochemist L. J. Henderson once stated (Hemoglobin,
4). However bold this statement seems, it must be realized that hemoglobin is, at least
in the scientific world, by far the most studied and most discussed substance in the
human body, as well as in other living organisms. Hemoglobin is the carrier in blood
that transports oxygen to our tissues and carbon dioxide out of our body, changing colors
as it does so. Hence, hemoglobin has long been termed the pigment of our blood.
Hemoglobin was one of the first proteins to be purified to the point where its molecular
weight and amino acid composition could be accurately measured. This finding was very
important in that it eventually lead to the understanding that a protein is a definite
compound and not a colloidal mixture of polymers. Each molecule was built from exactly
the same amino acid subunits connected in the same order along a chain,
and had exactly the same weight. Most organisms have their own unique, individual chain
of proteins to make up their hemoglobin, but all organisms share certain similarities, so
striking that they are unable to be ignored. Let's take, for example, the first
twenty-five amino acids in the alpha hemoglobin chains of 7 different animals: a human
man, rhesus monkey, cow, platypus, chicken, carp (bony fish), and shark (cartilaginous
fish) (See Table 1.1.) As is shown, the variations increase the further apart the
organisms are on the proposed evolutionary scale. A human man differs from a rhesus
monkey only twice in the first twenty-five amino acids of their alpha hemoglobin chains,
whereas a man and a cow differ in three areas. This is the product of many thousands of
years of natural fine tuning, if you will, through the slow but precise processes of
natural selection and adaptation. The fact of natural selection shows us that while most
genetic mutations usually prove fatal, a slim few are actually benef
icial, and assist the mutant in living and procreating offspring. This assistance helps
the mutant-gene's frequency grow in the gene pool and remain there since all progeny
possessing this certain trait are going to have an advantage over the other organisms
lacking this quality. This is the basis for evolution. The higher a certain species is
on the evolutionary scale, the more advanced that organism is due to a slight change in
the amino acid sequences of certain genes. An example would be that of the human man,
the rhesus monkey, and the cow. There is a smaller difference in the amino acid
sequences between a man and a monkey than between a man and a cow, and, respectively, a
monkey is more advanced than a cow, genetically (monkeys and humans have far advanced
apposable thumbs.) Also, where the amino acids have been conserved between all the
studied organisms, such as in columns 27, 31, and 39, indicates that in order for the
species to survive, that certain amino acid must be there. If it is changed
in any way, the organism can not survive. There are thirty-four conserved positions in
the first 141 amino acids in the seven studied organisms. After just these few
demonstrations, how could anyone doubt the theory of evolution? This question leads me
into a short interlude where I will discuss the arguments on both sides, and show just
how endless this debate could be.
Evolution -vs- Creation: Which Is Truth?
When evolution is mentioned to many people, the first thing that enters their mind is
the completely incorrect thought that man evolved from monkeys. Man did not, in fact,
evolve from monkeys, this is a known and agreed upon fact. The only connection between
modern day men and modern day chimpanzees, for example, is the fact that they must have
shared a common ancestor. The "common ancestor" theory, as I have chosen to name it,
states that all life living, or ever to have lived on this planet can be traced back to a
single, common ancestor. At some point in time, between 3.5 and 4.1 billion years ago, a
certain grouping of chemicals came together at just the right time and life began. From
this single life-form, the slow process of natural selection began. First came the
proteinoid microsphere, the first organisms on the planet to carry on all life functions.
Eventually, then, came viruses, parasites, saprophytes, holotrophs, chemosynthesizers,
and photosynthesizers, all mutants of the very first cell.
Some have tried to use thermodynamics to disprove evolution, especially the second law.
The second law of thermodynamics states that "all energy transfers or transformations
make the universe more disordered." These speculatives claim that since man is more
advanced than any other creature, we are more ordered. This is wrong. Man is more
advanced due to the mutations in his genes. Compared to the very first life-form's
genes, a human man's amino acid sequences are very dissimilar, or more disordered. Also,
the first law of thermodynamics can be used for either argument. The first law of
thermodynamics states that energy cannot be created or destroyed--in other words it has
always been here. Using this law, the matter in the universe can either be thought of as
always being here, or that the creator, with his infinite power, simply transformed the
energy that he possessed into the matter of the universe. Both sides have an arguable
point that agrees with the laws of thermodynamics. Another arguable
point that is worthy of mention is the discrepancies in the fossil record. The Earth's
crust, and all the fossils contained therein, can also be utilized as arguments for both
sides. The "Pre-Cambrian Void" (Creation-Evolution: The Controversy, 362) shows very
little sign of fossilization. Then, suddenly, massive amounts of fossilization can be
found during the Cambrian times, pointing to some sort of catastrophe, like a flood. The
Bible mentions a flood sent by God to destroy every living thing on Earth. The fact that
a flood could have happened, in that sense, strengthens the creationists' views. The
evolutionist theory can use these facts in two ways. One, when the selection pressure on
a species is constant for a long time, a species could become so specialized that any
slight change in their environment could lead to extinction, this is called a climax
group. Around the time that the large amounts of fossilization was occurring, the Earth
had cooled down enough to allow the immensely dense atmosph
ere to condense, thus causing many years of rain. Would not this rain cause almost any
climax group's entire population to become extinct? Also, before the rains came, the
great majority of the organisms inhabiting the Earth were land creatures. Once the rains
came, the Earth was covered in water, killing thousands of populations, and effectively
burying them in the water. The water preserved their parts for fossilization. These
have been just a few double sided arguments demonstrating that either side can turn any
facts around to fit their own hypothesis.
Leghemoglobin, Protein Relations Between Species, and the Evolution of the Globin Family
Like animals, plants also carry a sort of hemoglobin, leghemoglobin. Leghemoglobin is a
globin which is less evolved than that of hemoglobin or myoglobin. The whole globin
family, itself, has undergone much evolution and mutation. At one time, animals had no
globin at all. As life evolved, a single-chain oxygen-binding substance formed--we will
call this the basic globin. Then life branched into two parts: animals carrying the
basic globin, such as annelid worms, insects, and mollusks, and creatures (manly plants)
carrying leghemoglobin, a mutation of the basic globin. The animal kingdom's globin
eventually split into myoglobin (Mb) and hemoglobin (Hb). Since then, myoglobin has
basically stayed the same in many organisms (See Table 1.3.) Hemoglobin, on the other
hand, has undergone some major mutations. After the basic globin bifurcated into Mb and
Hb, Hb split into alpha (a) and beta (b) chains. The a-chain eventually split into two
parts, and has remained this way up to present times. The b-cha
in split into many more parts. Everything that has been said up until now about the
evolution of the globins from a common single-chained oxygen-binding ancestor has been
summarized in Table 2. If one would compare sequences of globin between species, one
would notice that the less amino acids that are different the more closely related two
species are. If we used this theory on the vertebrates that were studied it would give
us a "schematic family tree of globin containing vertebrates" (Hemoglobin, 78) (See Table
3). This same tree is obtained by comparing sequences of myoglobin, or the a or b chains
of hemoglobin. This tree tells us that all organisms alive today are just as evolved as
any other living organism. Different species evolve in different ways, that is the basis
of evolution. Man is just as evolved as a chimpanzee, or a carp, or a rose bush.
Different organisms simply evolved differently. Another excellent way of showing the
relationships between organisms is the mean amino acid differenc
es. The more amino acids that are different between two species, the further apart they
are genetically. For instance, of the entire b-chain of human and rhesus monkey
hemoglobin, there are, on the average, eight places where the amino acids are different.
However, when comparing b-chains of man and platypus, there are thirty four average
differences. A chart and a graph can help us better understand these points (See Table
4.) The amino acids that have changed are a result of mutated DNA that has proven
beneficial to the carrier mutant. This process, as stated before, is the basis of
evolution.
Speaking solely of hemoglobin, the variances between species can be shown through
greater or less affinity for oxygen. "H. F. Bunn has shown that mammalian hemoglobin can
be divided broadly into two groups: the great majority have intrinsically high oxygen
affinity, which is lowered in the red cell by DPG," (D-2,3-biphosphoglycerate) "while
those of ruminants and cats (Cervidae, Bovidae, Felidae) and of one primate, the lemur,
have an intrinsically low oxygen affinity that is little, or not at all, lowered by DPG
("Species Adaptation in a Protein Molecule", 16). DPG is one of the ligands that
"reduces the oxygen affinity of hemoglobin in a physiologically advantageous manner by
combining preferentially with the T structure." ("Species Adaptation in a Protein
Molecule", 3) For instance, the mole (Talpa europaea) lives in its burrows under
conditions lacking a rich oxygen supply. This creatures hemoglobin has adapted to having
a high oxygen affinity, a high concentration per unit volume of blood, and a lo
w body temperature. This high affinity is due to the mole's hemoglobin's low affinity
for DPG. So as you can see, DPG asks as a type of buffer. The more DPG the creature's
hemoglobin can hold, the less space it has for oxygen. Since the environment has low
amounts of oxygen, the blood needs to hold as much oxygen as possible, so the mole has
adapted.
Which Came First?
One final point that should be mentioned is the question of which change came first.
Did a mutation occur that adapted a species to a new environment take place before the
species occupied that environment, or did the genetic change occur after the environment
changed in order to assist the creature with living in its new surroundings? "W. Bodmer
suggested that once a large change in chemical affinities produced by one mutation had
enabled a species to occupy a new environment, its effect might have been refined by
later adaptive mutations, each contributing minor shifts, over a long period of time."
("Species Adaptation in a Protein Molecule", 22.) For example, did a llama's hemoglobin
adapt to a higher grazing altitude by increasing the oxygen affinity, or did the oxygen
affinity increase and the llama then realize that it could graze higher than some other
animals. This could show the "punctuated equilibria" (Biology, 296) in the evolution of
a species.
What Does It All Mean?
After seeing all of these demonstrations of adaptation at a molecular level, you may ask
what it adds to the betterment of the world. The truth is, merely knowledge. It is
doubtful that the evolution-creation controversy will ever be settled, but without
interest, research, and work by people in all corners of the debate, be it theological,
or scientific, the answer will never be discovered. It is quite possible that neither
hypothesis is correct--perhaps the truth lies in a combination of the two, or something
completely different. I believe that the truth, at least a partial truth, can be found
somewhere at the molecular level. If the genes, and amino acid sequences are examined, I
believe that the actual evolutionary time table can be reconstructed. The human species,
however, must be the last "stem" on this branch of the evolutionary tree, due to our
personal views of mutations. We all see mutations as negative, when some may actually be
positive. If a child is born with twelve fingers instead of
ten, two are surgically removed, and the child becomes less attractive to the opposite
sex, and may not get his mutated genes back into the gene pool. This process has almost
always worked in the opposite way in every species up until now--the mutant with the
beneficial, but different, genotype and (perhaps) phenotype has had an advantage that
makes him more attractive to the opposite sex, and his genes are passed on to his
offspring. One of the only mutations that could, and has, gone unnoticed is the
expansion of the control of the mind. Over the hundreds of years of human existence,
especially in the past few decades, the knowledge of the modern man has expanded
dramatically, and now, with the ease of the internet, anyone can learn about anything
imaginable. People are tired of mind-numbing thoughtless hours spent in front of the
television, and are now expanding their minds in their free time. I can only hope that
this paper has inspired some thought about the subject, and has brought us a small step
closer to the conclusion of the debate.
Works Utilized
Dickerson, Richard E. and Geis, Irving. Hemoglobin: Structure, Function, and
Pathology.
California: The Benjamin/Cummings Publishing Company Inc., 1983.
Perutz, Max. "Species Adaptation in a Protein Molecule", Molecular Biology and Evolution
Chicago: University of Chicago, 1983
Mammrack, Mark. Biology 112 Lecture Notes. Ohio: Wright State, 1996
Wysong, Randy. The Creation-Evolution Controversy. Michigan: Inquiry Press, 1978
Lasker, Gabriel. Human Evolution. New York: Holt, Rinehart, and Winston, Inc., 1963
Campbell, Neil. Biology Second Edition. California: The Benjamin/Cummings Publishing
Company Inc., 1990
Solomon, Eldra; Berg, Linda; Martin, Diana; Villee, Claude. Biology Fourth Edition. New
York: Saunders College Publishing, 1996
Genbank. National Center for Biotechnology Information, 1996
Available www: http://www2.ncbi.nlm.nih.gov/cgi-bin/genbank
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